reverse transcription pcr meaning in English

逆转录pcr技术

First , mrna was purified from the total rna extracted from fresh spleens of nonimmunized mice of kunming , then the cdna library was achieved via reverse transcription pcr . gene fragments encoding v
首先从未免疫小鼠的脾脏中提取总rna ，分离出mrna ，经逆转录合成其总cdna 。
The aim of this study was to investigate the differential expression of mpges and cpges in mouse uterus during early pregnancy and its regulation under different conditions by in situ hybridization and immunohistochemistry . mpges expression in the preimplantation mouse embryos was also performed by reverse transcription pcr ( rt - pcr )
本实验首先利用原位杂交和免疫细胞化学的方法，检测小鼠正常妊娠1 - 8天子宫中mpges和cpges的表达情况，并利用假孕、延迟着床和人工诱导蜕膜化等模型研究mpges和cpges的表达及其调节。